Composite

Part:BBa_K3790237:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-18)


GFP controlled by pBAD/araC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1885
    Illegal SapI site found at 961


Design Notes

The following primers were used for constructing this part.

f_pET_dps1	5-GATGTCTGCCTGTTCATCCG
f_pET_dps2	5-CACATAGCCGGTGCTATACTTAATCTCGGAATTGTGAGCGGATAACAATTCC
f_pET_GFP	5-GATATACCATGGCTGAGGGATGCGTAAAGGAGAAGAACTTTTCAC
r_pET_dps1	5-GTATAGCACCGGCTATGTGTTCCGCTATTCATTTCGCGGGATCGAGATC
r_pET_dps2	5-CGGATGAACAGGCAGACATC
r_pET_GFP	5-CAGCCGGATCCTTATTTCGCTTATTTGTATAGTTCATCCATGCCATGTGT
f_pBAD_dps1	5-CAGCTGTCCCTCCTGTTCAG
f_pBAD_dps2	5-CACCGGCTATGTGTTCCGCTATTCggatccgctaatcttatggataaaaatgc
f_pBAD_GFP	5-ggtgagccagtgtgaATGCATGCGTAAAGGAGAAGAACTTTTCAC
f_pBADtran_tem	5-CAGACATtttttttacctccttaactctagtagctag
r_pBAD_dps1	5-GCGGAACACATAGCCGGTGCTATACTTAATCTCcatacccgtttttttgggctagctac
r_pBAD_dps2	5-CTGAACAGGAGGGACAGCTG
r_pBAD_GFP	5-ggaggtaaaaaaaATGTCTGTTATTTGTATAGTTCATCCATGCCATGTGT
r_pBADtran_tem	5-GCATtcacactggctcaccttc



Source

References